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Cell Culture Flasks, Plug Seal Caps

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NEST Cell Strainers enable rapid and efficient filtration of cell suspensions. Available in multiple mesh sizes for sample preparation and single-cell isolation.

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Image	SKU	Cell Growth Area	Maximum Graduated Volume	LWH	Package	TC/Sterile	Availability	Price	Quantity	Add to cart
	727011	T400	1200 mL	280.5203.356.4 mm	Bag Package, 2 pcs/pack, 10 packs/case, 20 pcs/case	Non-TC / Sterile	Out of stock	0,00 €	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list
	727001	T400	1200 mL	280.5203.356.4 mm	Bag Package, 2 pcs/pack, 10 packs/case, 20 pcs/case	TC / Sterile	Out of stock	0,00 €	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list

Precautions:

It is important to handle multi-layer Cell Culture Flasks with caution to avoid the formation of bubbles. The presence of bubbles can lead to the creation of siphon bridge at the baffle, resulting in the upper layer of culture medium flowing down to the bottom.

Feature

Cell Culture Flask

Made of high clarity, 100% virgin polystyrene.

Sterilized by E-beam, SAL=10-6.

Non-Pyrogenic, DNase/Rnase free.

Frosted writing and clear graduations.

Notched bottom for slip free slip stacking.

Clear lot number for batch traceability.

Individually packaged in sterile bag.

3/ 5 - Layer Cell Culture Flask

Made of high clarity, 100% virgin polystyrene.

Sterilized by E-beam, SAL=10-6.

Non-Pyrogenic, DNase/Rnase free.

3-Layer Cell Culture Flask Growth area: 520 cm².
5-Layer Cell Culture Flask Growth area: 870 cm².

Individually packaged in sterile bag.

Product Details

Flasks feature frosted writing areas for easy culture identification
Each flask has a clear lot number printed on each flask for traceability
Flasks bottoms are designed with raised edges for slip free stacking

Quality Details

Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products

Tissue culture flasks are engineered for the cultivation and maintenance of mammalian, microbial, or insect cell cultures. They are particularly well-suited for maintaining seed cultures, which can be used to inoculate tissue culture plates and dishes. This model features a plasma-etched surface that supports optimal growth of adherent cell lines.

FAQs- Tissue Culture Flasks

What does “TC treated” mean?

TC (Tissue Culture) treatment refers to a surface modification that enhances cell attachment. The flask surface is treated to make it more hydrophilic, allowing adherent cells to spread and grow more efficiently.

What is the difference between plug seal and vented caps?

Plug seal caps provide a tight, closed system when fully tightened, minimizing contamination and evaporation.
Vented caps allow gas exchange (CO₂/O₂) without loosening the cap.
**Plug seal caps can still allow gas exchange when slightly loosened**

Are these flasks sterile and ready to use?

Yes the are supplied sterile and ready for immediate use. Nest Scientific utilizes advanced

Manufacturer Cross Reference

Tissue Culture Flask Manufacturer Cross Reference

Nest SKU	Size / Cap Type	Corning/Falcon	Nunc	Genesee	Greiner	VWR	CellTreat	TPP	Surface Treatment
707001	T25, Plug Seal	430168 & 353014	156340	25-206	690160	10062-874	229330	90025	TC Treated
707003	T25, Vent Cap	430639, 353109 & 353108	156367	25-207	690165	10062-872	223331	90026	TC Treated
707011	T25, Plug Seal	430639 & 353009	NA	25-213	690160	10861-570	N/A	N/A	Non-Treated
707013	T25, Vent Cap	3289 & 431463	169900	N/A	690195	10861-572	229500	N/A	Non-Treated
708001	T75, Plug Seal	353133 & 353135	156472	25-208	658170	10062-362	229340	90075	TC Treated
708003	T75, Vent Cap	430720U & 353133	N/A	25-209	658175	10062-862	229341	90076	TC Treated
708011	T75, Plug Seal	430720U & 353133	N/A	25-214	658190	10861-574	N/A	N/A	Non-Treated
708013	T75, Vent Cap	431464U	156800	N/A	658195	10861-576	229510	N/A	Non-Treated
709001	T175, Plug Seal	431079 & 355000	159920	25-210	660160	10062-866	229351	90150	TC Treated
709003	T175, Vent Cap	431080 & 355001	159910	25-211	660175	10062-864	229351	90151	TC Treated
709011	T175, Plug Seal	N/A	N/A	N/A	660190	10861-582	N/A	N/A	Non-Treated
709013	T175, Vent Cap	3292 & 431466	159926	25-215	N/A	10861-584	229520	N/A	Non-Treated
721001	T225 Plug Seal Cap	431081 & 353139	159933	N/A	N/A	78112-960	229370	90300	TC Treated
721003	T225聽聽Vent Cap	431082 & 353138	159934	N/A	N/A	78112-962	229371	90301	TC Treated
721011	T225 Plug Seal Cap	N/A	N/A	N/A	N/A	N/A	N/A	N/A	Non-Treated
721013	T225 Vent Cap	3293	N/A	N/A	N/A	N/A	229530	N/A	Non-Treated

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Multi-Layer Cell Flasks Manufacturer Cross Reference

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General FAQ

What countries does NEST Scientific Shop ship to?

NEST Scientific Shop provides shipping services across Europe. Some countries or regions in EU may have limited delivery availability due to logistics or regulatory requirements. Please contact us if you need confirmation before placing an order.

How long does delivery take?

Orders are typically delivered within 5-7 business days after shipment. Delivery time may vary depending on the destination, product availability, and local logistics conditions.

Do you provide support for bulk or distributor orders?

Yes. NEST Scientific Shop supports bulk orders and distributor cooperation. Please contact our sales team for customized pricing, product availability, and delivery arrangements.

What should I do if a product is out of stock?

If a product is unavailable, you can add it to your quote list and send us. Our team will contact you with availability updates and possible supply solutions.

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Nest SKU	Multi-Layer Flask	Cap Type	Cell Treat	Genesee	Falcon	Nunc	VWR	Use Case
731301	3 Layer TC Treated	Plug Cap	N/A	N/A	N/A	132867	62407-079	Controlled handling, short-term culture, and transport-sensitive workflows
731302	3 Layer TC Treated	Vent Cap	229323	25-150	353143	132913	62407-082	Standard expansion and routine adherent cell culture
731001	5 Layer TC Treated	Plug Cap	N/A	N/A	N/A	N/A	N/A	High-density culture with controlled, low-exchange conditions
731002	5 Layer TC Treated	Vent Cap	229325	25-151	353144	N/A	89204-478	Large-scale adherent cell production and biomanufacturing workflows

Cell Culture Plates, Flat Bottom

Microcentrifuge Tubes

Serological Pipettes

15/50 mL Centrifuge Tubes, Standard Level

Vacuum Filtration System

Pipette Tips Racked, Sterile