Cell Culture Inserts, Tissue Culture Treated, PC/PET Memberane
Cell culture inserts are widely used in cell experiments, such as co-culture, chemotaxis, cell migration, invasion, and drug transport studies. The permeable support can effectively improve the culture of polarized cells, as it allows secretion and absorption of molecules from both the basal and apical surfaces, thereby supporting metabolism in a more natural manner. This enables the cultivation of certain specialized cell lines while closely mimicking the in vivo environment.
Price range: 10,00 € through 40,00 €
| SKU | Pore Size | Description | Membrane Type | Price | Quantity | |
|---|---|---|---|---|---|---|
| 724101 | 0.4 μm | 12 Well Plates+12 Inserts | PC Membrane | 10,00 € | ||
| 725301 | 8 μm | 24 Well Plates+12 Inserts | PC Membrane | 20,00 € | ||
| 723121 | 0.4 μm | 6 Well Plates+6 Inserts | PET Membrane | 20,00 € | ||
| 725121 | 0.4 μm | 24 Well Plates+12 Inserts | PET Membrane | 30,00 € | ||
| 725321 | 8 μm | 24 Well Plates+12 Inserts | PET Membrane | 40,00 € |
Feature
Product Details
PC membrane inserts are ideal for tumor cell migration and invasion studies, hematostaxis or endothelial cell migration assays and co-culture applications.
Create a cell culture environment that closely mimics in vivo conditions.
Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides
Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact
Treated to promote maximum cell adhesion
Membrane requires staining to be visible
The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds
PC is compatible with common solvents used for fixation and staining
Create a cell culture environment that closely mimics in vivo conditions.
Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides
Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact
Treated to promote maximum cell adhesion
Membrane requires staining to be visible
The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds
PC is compatible with common solvents used for fixation and staining
Quality Details
USP grade VI virgin polystyrene
USP grade VI virgin polycarbonate
Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products
USP grade VI virgin polycarbonate
Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products
Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.
Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.
Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.
Application Directions and Types
| Applications | Cells | Pore Size |
| ADME (compound transport across intestinal epithelial cell monolayers,permeability assays) | Caco-2, MDCK | 0.4 μm |
| Co-culture and cell differentiation, cell imaging | Primary cells, stem cells | 0.4 μm, 1.0 μm |
| Macromolecule or virus transport and separation | 1.0μm, 3.0 μm | |
| Cell migration and invasion (angiogenesis), cell-cell interactions (migration) or trans-endothelial migration; chemotaxis assays; co-culture | Endothelial cells, leukocytes, neurons | 3.0 μm |
| Cell migration and invasion | Macrophages, giant cells | 3.0 μm, 5.0 μm |
| Tumor cell migration and invasion; leukocyte-endothelial cell interaction; endothelial cell proliferation; co-culture | Tumor cells, leukocytes, tumorstem cells, MSCs | 8.0 μm |
Cell Culture Inserts(Insert+Plate Configuration)
| Pore Size (μm) | Description | Packaging | PC Membrane | PET Membrane | ||||
| Transparence | TC Treated | Non-Treated | Transparence | TC Treated | Non-Treated | |||
| 0.4 | 6 Cell Culture Inserts + 6 Well Plate | 4 | / | / | / | Opaque | 723121 | / |
| 12 Cell Culture Inserts + 12 Well Plate | 4 | Opaque | 724101 | / | / | / | / | |
| 12 Cell Culture Inserts + 24 Well Plate | 4 | / | / | / | Opaque | 725121 | / | |
| 8 | 6 Cell Culture Inserts + 6 Well Plate | / | / | / | / | / | / | / |
| 12 Cell Culture Inserts + 12 Well Plate | / | / | / | / | / | / | / | |
| 12 Cell Culture Inserts + 24 Well Plate | 0.1-0.2 | Translucent | 725301 | / | Transparent | 725321 | / | |
Product Specification
Cell Culture Inserts
| Specifications | Insert(Pcs/Plate) | insert Diameter(mm | Volume of Each Well(mL) | Inner Volumeof Insert(mL) | MembraneMembrane Growth Area of Insert(cm²) |
| 6 Well Plate | 6 | 24 | 2.6 | 1.5 | 4.67 |
| 12 Well Plate | 12 | 12 | 1.5 | 0.5 | 1.12 |
| 100mm Dish | 12 | 6.5 | 6.5 | 0.1 | 0.33 |
| 8 | 1 | 75 | 7.5 | 9 | 44 |
| Pore Size of Membrane (μm) | 0.4 | 1.0 | 3.0 | 5.0 | 8.0 |
| Membrane Density (Well/cm²) | 1x10^8 | 2x10^7 | 2x10^6 | 4x10^5 | 1x10^5 |
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