Name: Vacuum Filtration Systems, Filter Tops, PES Membrane
Availability: InStock

Feature

High flow rates and fux.

Low protein binding and low
chemical extractability.

The configuration complies with the ANSI-SBS format.

Pore size: 0.22 μm.

Non-Pyrogenic, DNase/RNase free.

Sterilized by E-beam, SAL=10-6.

Endoxin free,pyrogen free.

For laboratory use only.

If the package is damaged, pleasedo not use.

Membrane Type	Hydrophilicity	Features	Application Directions	Cautions
PES	Hydrophilic	Low protein adsorption, high fl ow rate, wide PH range, high chemical compatibility, good heat resistance.	For general culture media and aqueous solutions, polar or middle-polar solvents, neutral aqueous solutions.	Not applicable for chloroform, esters, amides and strong acids or strong bases.
PVDF	Hydrophilic	Wide practicability, good oxidation resistance and heat resistance.	May be used for aqueous solutions and most solvents, including strong non-polar solvents. Ideal for preparations of HPLC and GC.	Not applicable for strong acids and bases.
MCE	Hydrophilic	Strong chemical compatibility and low protein adsorption, the optimum pH range is 3-6.	Used for particle analysis of general culture media and aqueous solutions. HPLC sample preparation.	The set should not be used for filtration of ethanol and alkaline solutions. The working temperature should not be over 40°C.
CA	Hydrophilic	High flow rate and heat stability as well as very low adsorption, stable within the range of pH 4-8	It may resist most alcohols and oils, and is suitable for sterling filtration of aqueous solutions, buffers, serum and culture media, as well as filtration of the moving phase of HPLCD.	The CA membrane may accommodate a smaller volume of buffer since it has low hydrophilia.

Quality Details

Low protein binding
Not for use with chloroform, esters, amides, strong acids or strong bases
High flow rates and high throughput
Ideal for sterilizing and clarifying cell culture media
Hydrophilic
45℃ maximum working temperature
50mm diameter membrane filter
Polystyrene cap and cup on top
Acrylonitrile-Butadiene-Styrene (ABS) neck

Quality Details

Low chemical extractables
Produced in a cGMP (21 CFR part 820) ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
Sterilized by E-beam, SAL=10-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA\Lot tested free BSE/TSE
Certified free of animal products

Vacuum bottle top filter systems provide a cost-effective and convenient solution for sterilizing and clarifying water-based biological media. Equipped with a PES membrane, they deliver fast flow rates and minimal protein binding. This model includes a pre-attached polystyrene receiving bottle for added ease of use. Ideal for routine filtration tasks where high chemical resistance is not required.

FAQs- PES Bottle Top Filters

What are NEST PES bottle top filters used for?

NEST PES bottle top filters are designed for vacuum-driven filtration of aqueous solutions in laboratory settings. Common applications include sterile filtration of cell culture media, buffer preparation, clarification of solutions, and removal of particulates or microbial contamination

What is the difference between 0.10 µm, 0.22 µm, and 0.45 µm filters?

0.10 µm:High-retention filtration for mycoplasma reduction and fine particle removal
0.22 µm:Industry standard for sterile filtration of media and reagents
0.45 µm:Clarification and pre-filtration to remove particulates and debris
Choosing the correct pore size depends on whether you need clarification, sterilization, or maximum contamination control.

Why use a PES membrane?

PES (polyethersulfone) membranes offer:
Low protein bindingfor maximum sample recovery
High flow ratesfor faster filtration
Low extractablesfor sensitive applications
This makes PES ideal for cell culture, biologics, and pharmaceutical workflows.

What is the difference between filter-only units and filter & bottle systems?

Filter & bottle systems:Include both the filter unit and a sterile receiver bottle for complete workflows
Filter-only units:Designed to be used with existing bottles or vacuum setups
Filter-only options are more cost-effective for labs that already have compatible collection bottles.

Are NEST bottle top filters sterile?

Yes. NEST bottle top filters are:
Sterilized (SAL 10⁻⁶)
DNase/RNase-free
Non-pyrogenic
They are ready for immediate use in sterile workflows.

What types of solutions can be filtered?

These filters are suitable for:
Cell culture media
Buffers and aqueous solutions
Serum and supplements
Protein and antibody solutions
They are not recommended for organic solvents unless compatibility is confirmed.

What size should I choose: 250 mL, 500 mL, or 1000 mL?

250 mL:Small-volume or high-value samples
500 mL:Routine lab use
1000 mL:Large batch preparation and high-throughput workflows
Select based on your typical working volume and throughput needs.

Are these filters compatible with standard vacuum systems?

Yes. NEST bottle top filters are designed to fit standard laboratory vacuum filtration setups and tubing connections, making them compatible with most lab equipment.

How do NEST filters compare to other brands?

NEST PES bottle top filters are comparable to leading brands such as Corning, Thermo Fisher Scientific, and Sartorius in terms of:
Filtration efficiency
Flow rate
Sterility assurance
They provide a cost-effective alternative without sacrificing performance.

When should I use a 0.45 µm filter before a 0.22 µm filter?

Use a 0.45 µm filter as a pre-filtration step when working with:
Viscous solutions
High particulate loads
Serum-containing media
This helps prevent clogging and extends the life of the 0.22 µm filter.

Can these filters be used for sterile filtration of cell culture media?

Yes. The 0.22 µm PES filters are specifically designed for sterile filtration and are widely used for preparing cell culture media and reagents.

Do these filters bind proteins or reduce yield?

PES membranes are known for low protein binding, which helps maximize recovery of valuable samples such as antibodies, enzymes, and other biologics.

Vacuum Filtration System

Volume(mL)	Pore Density (μm)	Packaging	PackagingVacuum Filtration Systems (Sterile)1/Pack, 12/Case
Volume(mL)	Pore Density (μm)	Packaging	PES Memberance
250	0.22	Bag Package	342001
500	0.22	Bag Package	343001
1000	0.22	Bag Package	344001

Technical Drawing of Erlenmeyer Flasks

Manufacturer Cross Reference

Nest SKU	Bottle Top Filter	Type	Cell Treat	Corning	Fisher	Nalgene	VWR	Use Case
344002	1000 mL Vacuum Filter System, 0.10 um, PES, Sterile	Filter & Bottle	229724	431474	N/A	567-0010	N/A	High Retention / Fine Filtration, Mycoplasma reduction and fine bioburden control
344001	1000 mL Vacuum Filter System, 0.22 um, PES, Sterile	Filter & Bottle	229708	431098	FB12566506	567-0020	10040-440	Standard Sterile Filtration, Sterile filtration of cell culture media
344003	1000 mL Vacuum Filter System, 0.45 um, PES, Sterile	Filter & Bottle	229704	N/A	FB12566507	167-0045	10040-442	Clarification / Pre-Filtration, Clarification of cell culture media before sterile filtration
344011	1000 mL Vacuum Filter, 0.22 um, PES, Sterile	Filter Only	229718	431174	FB12566512	N/A	10040-472	Standard Sterile Filtration, Sterile filtration of cell culture media
343002	500 mL Vacuum Filter System, 0.10 um, PES, Sterile	Filter & Bottle	229702	431475	N/A	566-0010	N/A	High Retention / Fine Filtration, Mycoplasma reduction and fine bioburden control
343001	500 mL Vacuum Filter System, 0.22 um, PES, Sterile	Filter & Bottle	229706	431097	FB12566504	566-0020	10040-436	Standard Sterile Filtration, Sterile filtration of cell culture media
343003	500 mL Vacuum Filter System, 0.45 um, PES, Sterile	Filter & Bottle	229703	N/A	FB12566505	169-0045	10040-438	Clarification / Pre-Filtration, Clarification of cell culture media before sterile filtration
343011	500 mL Vacuum Filter, 0.22 um, PES, Sterile	Filter Only	229717	431117 & 431118	FB12566510	N/A	10040-468	Standard Sterile Filtration, Sterile filtration of cell culture media
342002	250 mL Vacuum Filter System, 0.10 um, PES, Sterile	Filter & Bottle	229722	N/A	N/A	568-0010	N/A	High Retention / Fine Filtration, Mycoplasma reduction and fine bioburden control
342001	250 mL Vacuum Filter System, 0.22 um, PES, Sterile	Filter & Bottle	229706	431096	FB12566502	568-0020	10040-464	Standard Sterile Filtration, Sterile filtration of cell culture media
342003	250 mL Vacuum Filter System, 0.45 um, PES, Sterile	Filter & Bottle	229702	N/A	FB12566503	168-0045	10040-466	Clarification / Pre-Filtration, Clarification of cell culture media before sterile filtration
342011	250 mL Vacuum Filter, 0.22 um, PES, Sterile	Filter Only	229716	N/A	N/A	N/A	10040-448	Standard Sterile Filtration, Sterile filtration of cell culture media

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