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SKU	Pore size	Specification	Transparence	Package	TC/Sterile	Availability	Quantity	Add to cart
725122	0.4 μm	24 Well	Opaque	Blister Package, 1 pc/pack, 8 pcs/plate, 6 plates/case, 48 pcs/case	TC / Sterile	Out of stock	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list
724122	0.4 μm	12 Well	Opaque	Blister Package, 1 pc/pack, 8 pcs/plate, 6 plates/case, 48 pcs/case	TC / Sterile	Out of stock	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list
723122	0.4 μm	6 Well	Opaque	Blister Package, 1 pc/pack, 8 pcs/plate, 6 plates/case, 48 pcs/case	TC / Sterile	Out of stock	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list
725302	8 μm	24 Well	Translucent	Blister Package, 1 pc/pack, 8 pcs/plate, 6 plates/case, 48 pcs/case	TC / Sterile	Out of stock	Max: Min: 1 Step: 1	Add to quote Add to quote See quote list

Product Specification

Cell Culture Inserts

Specifications	Insert(Pcs/Plate)	insert Diameter(mm	Volume of Each Well(mL)	Inner Volumeof Insert(mL)	MembraneMembrane Growth Area of Insert(cm²)
6 Well Plate	6	24	2.6	1.5	4.67
12 Well Plate	12	12	1.5	0.5	1.12
100mm Dish	12	6.5	6.5	0.1	0.33
8	1	75	7.5	9	44

Pore Size of Membrane (μm)	0.4	1.0	3.0	5.0	8.0
Membrane Density (Well/cm²)	1x10^8	2x10^7	2x10^6	4x10^5	1x10^5

Feature

Innovative edge design: easy sample addition.

PC membrane: low-binding, minimizing the loss of small proteins and other compounds.

The configuration complies with the ANSI-SBS format.

PET membrane: superior optical clarity, facilitating observation of cell status.

Compatible with most solvents used in fixation and staining.

Product Details

PC membrane inserts are ideal for tumor cell migration and invasion studies, hematostaxis or endothelial cell migration assays and co-culture applications.
Create a cell culture environment that closely mimics in vivo conditions.
Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides
Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact
Treated to promote maximum cell adhesion
Membrane requires staining to be visible
The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds
PC is compatible with common solvents used for fixation and staining

Quality Details

USP grade VI virgin polystyrene
USP grade VI virgin polycarbonate
Manufactured in cGMP (21 CFR part 820/821) compliant factory
Produced in ISO 13485:2016 and ISO 9001 facility
Molded and packed in ISO 7 cleanroom
E-beam sterilized and lot tested to SAL 10^-6
Lot tested free of pyrogens USP 85
Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
Lot tested free DNase/RNase and DNA
Lot tested free BSE/TSE
Certified free of animal products

Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.
Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.

Application Directions and Types

Applications	Cells	Pore Size
ADME (compound transport across intestinal epithelial cell monolayers,permeability assays)	Caco-2, MDCK	0.4 μm
Co-culture and cell differentiation, cell imaging	Primary cells, stem cells	0.4 μm, 1.0 μm
Macromolecule or virus transport and separation		1.0μm, 3.0 μm
Cell migration and invasion (angiogenesis), cell-cell interactions (migration) or trans-endothelial migration; chemotaxis assays; co-culture	Endothelial cells, leukocytes, neurons	3.0 μm
Cell migration and invasion	Macrophages, giant cells	3.0 μm, 5.0 μm
Tumor cell migration and invasion; leukocyte-endothelial cell interaction; endothelial cell proliferation; co-culture	Tumor cells, leukocytes, tumorstem cells, MSCs	8.0 μm

Downloads

Cell Inserts Datasheet

Datesheet